VariantFind™ is a flexible and scalable PCR-based platform for creating DNA libraries for any gene of interest.
Our platform is not limited by target gene length or GC% composition, and all variants are generated by targeted mutagenesis approach.
VariantFind™ is the most cost-effective and time-efficient solution in the industry for all the high-throughput screening needs.
The VariantFind™ platform is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process. These oligonucleotides are combined in a series of sequential PCRs that result in a ready-to-clone DNA library encoding all desired mutations.
High-throughput design of mutagenic oligonucleotides using in-house software.
1. Retrieve sequence of gene target
2. Input into in-house design algorithm
3. Obtain mutagenic oligo sequences
Generate variant library of a target gene using a series of proprietary PCR processes.
Our DNA variant libraries are validated using a Sanger and/or Next-generation sequencing. All DNA libraries are cloned into an in-house vector and randomly selected clones are sent for Sanger sequencing. We ensure that each colony carries a unique variant. Additionally, libraries can be sent for Illumina deep sequencing to detect all variants created during the library building process.
Pricing starts at $45.00 USD per target amino acid
DNA libraries are shipped in 4-6 weeks
300 - 400ng of DNA is delivered in linear double stranded format
Libraries are screened by sequencing to ensure quality